Objectives: Our study was aimed to select the best growth factors cocktail for differentiation of MSCs into hepatocytes like cells (HLCs) in absence and presence of 3D microenvironment. Methods: Ninety ml BM was aspirated from the iliac bone for separation of MSCs. This study was conducted on 20 patients with advanced liver cirrhosis. They were divided into 2 groups. Group I; MSCs were plated onto 96-wellplates without microenvironment (control group). Group II; MSCs were plated onto 96-wellplates with 3D microenvironment. Surface expression of CD271, CD29 and CD34 were analysed using flowcytometry. MSCs were differentiated in-vitro into HLCs by adding 4 growth factors cocktails in presence and absence of 3D microenvironment. Hepatogenesis was assessed by immunohistochemical analysis of OV6, AFP albumin and cytokeratin 18 expressions. Results: there was statistically significant increase in the expression of CD271 and CD29 after MSCs culture (P <0.001). Heterogeneous cell population composed of MSCs and differentiated HLCs were encountered (40% hepatocytes and 60% MSCs). Furthermore our results showed that growth factor cocktails one and two gave the best result for differentiation of MSCs into HLCs. Conclusion: MSCs could be feasible, readily available and novel source for differentiation of MSCs into HLCs for future therapeutic implication

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