Tick infestation poses a major threat to the lifestyle of livestock. The main tick species found in domestic animals of India is Rhipicephalus [Boophilus] microplus. Several plants have the ability to inhibit the growth of the ticks in domestic animals. The present study aimed to evaluate the acaricidal activity of Acaricidal activity of Eucalyptus essential oil based nanoformulations against Ticks. Eucalyptus oil nanoemulsion was formulated in 1:1 ratio. The formulated eucalyptus oilnanoemulsion was characterized by transmission electron microscopy and dynamic light scattering, Fourier Transformed Infrared Analysis . The nanoemulsion droplets were found to have a Z-average diameter of 55.67nm and were spherical in shape. Efficacy of eucalyptus based nanoemulsion against ticks were tested based on In vitro and In Vivo test. The histopathology of tick -treated and untreated nanoemulsion was analyzed.