Using oilseeds with high oil content can play a major role in biodiesel production. In this study, safflower oilseed (Isfahan cultivar) was evaluated and experimented as a biodiesel production feedstock because of its high oil content (30 %). The results showed that the Kinematic viscosity (4.27 mm2/s) of the produced biodiesel from sfflower oil was in the approved range of D6751 ASTM standard. It was also close to the viscosity of soybean methyl ester, but it was better than palm. Its flash point (173˚C) was higher than petroleum diesel and the range of ASTM D6751 standard. In addition, its cold flow properties were similar to soybean methyl ester and better than palm. The cetan index of the produced fuel was lower than cetan index of palm oil methyl ester, but it was nearly as high as the cetan index of soybean methyl ester. The distillation range of the produced fuel was 303.4-391.2 ˚C.

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