A research employing seed-originated germplasm collected from several places in Jammu sub-tropics, J&K (UT), was conducted between 2015 and 2021 in order to choose better strains of kagzi lime (Citrus aurantifolia Swingle). The objective was to identify genotypes with better fruit quality in terms of total soluble solids (˚Brix), acidity (%), peel thickness (cm), juice content (%), ascorbic acid (mg/100g of juice). The findings of the factor analysis showed that the KL3 and KL13 had the highest TSS (7.67 ˚Brix), KL1 and KL11 had the maximum acidity (6.79 %), KL1 and KL11 strain had the lowest peel thickness (1.44 mm), KL1 and KL11 had maximum juice content (46.00%), KL1, KL5 and KL11 had maximum ascorbic acid (23.50 mg/100 ml of juice). This study shows the need of choosing superior genotypes for enhanced fruit quality and output while shedding information on the genetic variety of kagzi lime germplasm in Jammu sub-tropics. The results of this study might be used to create breeding programs and encourage the growth of high-quality kagzi lime cultivation in the area.