The present study aimed at evaluating the isolate phages that were active against Pasteurella multocid and to characterize to explore its possibility as therapeutic and prophylactic agents. The lytic range of the phage was determined against several bacterial isolates. Studies on a phage growth curve and its survival rate with respect to temperature and pH were carried out. In-vivo study was conducted to explore the levels of phage in the liver, spleen, kidney, lung, and blood at 24 h post-administration.The isolated phage was confirmed asthe family Podoviridae. Their estimated genome sizes were about 21 kb. On restriction enzyme digestion only 7 fragments in ranges of 3000 to 10000bp were seen. Phage was stable 5-50oC and survival pH was 4-9. In the safety and therapeutic study challenged mice treated with phage was showing mild clinical signs and died one or two mice after 72 hrs. and the survival rate increased significantly (P≤0.05). In poultry, in the challenged group at end of 72 hrs., 7 birds survived in the subcutaneously treated group but in nasal route treatment, only 5 birds survived at the end of 72 hrs. and concluded a good effect on treating the mice infected with different P. multocida type B2, A1 strains.