Background: Metalloid arsenic (As) is a proven human carcinogen. The As (III), As (V), and inorganic oxyanions are the most prevalent As species observed in natural waters. While sodium arsenate (Na3 AsO4) and sodium arsenite (NaAsO 2) have higher solubilities in water than arsenic trioxide and arsenic acid (H3 AsO 4), respectively. \nObjective: Molecular characterization of arsenite oxidizing bacteria \nMethods: In this investigation, materials were collected from Mathura and Ghaziabad in north India and streaked on a chemically defined medium containing sodium arsenite shows the growth of two γ-Proteobacteria. \nResult: The two strains (AOB-GPI and AOB-MTI) were identified Gram Negative. Based on 16S rRNA gene sequence analysis strains AOB-GPI and AOB-MTI revealed highest homology of 98% - 99% to Cronobacter sakazakii and Acinetobacter sp. respectively. To the best of our knowledge the native bacterial isolates have potential to oxidize arsenite and are reported for the first time for the presence of arsenite oxidase (AroA, AroB) and arsenite transporter (arsB,ACR3) gene. Acinetobacter sp. strain (AOB-GPI) and Cronobacter sp. strain (AOB-MTI) sequences were determined, and they were deposited in the Gene-Bank under accession numbers KR069103 and KR069104, respectively. Under aerobic circumstances, the stain Acinetobacter sp. oxidised the arsenite at a concentration of 10 mM and 15 mM for Cronobacter sp. in 24 hours. Conclusion : Our research demonstrates that these two isolated strains can be employed as a bioremediation alternative to remove arsenic from arsenic-contaminated water.