Pseudomonas aeruginosa is the most common and ubiquitous pulmonary pathogen of human which cause successfully unscrupulous infections in the patients with suppressed immune system of every age group and considered as the first or second key pathogen in supreme researches. Majority of the mortality and morbidity was caused by this pathogen every year globally due to several virulence factors and higher rates of significant infections. The most lethal and persistent pathogen that caused the acute ventilator acquired pneumonia (VAP) disease is Pseudomonas aeruginosa. The Quorum sensing (QS) system is present in Pseudomonas aeruginosa which act as the regulator which regulates the expression of various virulence factors. The current study was established to detect the las R gene associated with acute pulmonary infections in Pseudomonas aeruginosa. The samples include secretions (Endotracheal tract) and Blood samples from patients of acute Ventilator associated pneumonia (VAP) were provided by Microbiology lab of GCU Lahore. Total 5 strains of P.aeruginosa were selected as most virulent strains after confirmatory test of antibiotic sensitivity and virulence assays. Later these pathogenic strains of P. aeruginosa were used for Ribotyping of gene lasR. PCR products results and sequence analysis results after BLAST at NCBI confirmed 100% presence of lasR in the genome of P.aeruginosa strains which are involved in multiple virulence factors and responsible for acute pulmonary infections. In the current study the contribution of lasR gene of pathogenic P.aeruginosa responsible for acute pulmonary infections was investigated.

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