Biosensors incorporating miniature and high-sensitivity cantilevers are increasingly popular nowadays as part of the evolution of the Lab-on-Chip platforms with micro and nano-dimensions. Even though these sensors are operated in non-isothermal environments, the temperature is often neglected in mathematical investigations. Besides, no single layer cantilevers are adequate for biosensor design. But the effect of temperature increased with the surface to volume ratio as it is likely to be more dominant in Nano-Electro-Mechanical-Systems. Insight of this result, the mathematical modelling includes temperature and the associated material properties. This paper proposes the use of a simple analytical approach to analyze the control of bi-metallic cantilevers using NEMS based sensing and actuating mechanisms.The prominent mathematical equation models were derived and solved using methodological approaches. There is a significant correlation between all the other works investigated and the multivariate statistical data that affects their functioning has been identified. The simulation results examined using FEA comparisons and experimental studies shows mathematical model\'s predictions are more than 20% accurate

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