Purpose: The aim of our in vitro study was to evaluate the effect of diode laser irradiation (λ=660 nm and λ=970 nm) on the intracanal dentin and their interference in the apical seal of filled root canals. Materials and methods: 48 human single rooted teeth were randomly assigned into 4 groups. Root canal preparation was done using ProTaper Universal rotary system up to F3. 2 % Sodium hypochlorite, 17 % EDTA and distilled water were used as irrigants. The laser irradiation was performed at the end of the traditional endodontic preparation and teeth were filled with gutta-percha and AH- plus. The apical leakage was report on mm. Results: Statistical analysis showed that the laser-treated groups had significantly less leakage in apical third than the control group. Conclusions: Certain lasers can help in removing the smear layer and debris and can modify the morphology of the root canal wall for better root canal sealing.\n Keywords: diode laser, apical leakage, Protaper rotary instruments

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