The seed quality attributes relates to seminal physical (biomass and size), physiological (germination and vigor), genetic and health. Agave salmiana Otto ex Salm-Dyck propagates mostly asexually, although each plant produces hundreds of viable seeds. The objective in this study was to evaluate the physical characteristics and physiological quality of A. salmiana seeds from two Mexican regions. Seeds were collected at the Estado de M�xico (central M�xico) and San Luis Potos� (central Highland plateau) states, M�xico. Seed humidity and 100 seed biomass did not vary (p ? 0.05) among seed collections. Nevertheless, seed width, and length were different (p ? 0.05) among collects. Seed germination start (60 h), maximum cumulative seed germination at 25 �C and in total darkness, and cumulative maximum seedling emergence (with an exception) did not significantly vary among sites. The Dickson (3.14 x 10-4 to 5.93 x 10-4) and slenderness indexes (? 6) showed seedling quality. This last index positively correlated with seedling size (R2 = 0.9979 and 0.9989; p ? 0.05). The physical quality, in contrast to the physiological quality in A. salmiana seeds depends from the harvesting region, with few character exceptions; but, regardless of their development site, A. salmiana seeds are a potential resource for obtaining plants.